An anti-glypican 3 antibody with modified sugar chains, more specifically, an anti-glypican 3 antibody lacking fucose is provided. The anti-glypican 3 antibody with modified sugar chains of the present invention may be produced by a process comprising introducing a nucleic acid encoding an anti-glypican 3 antibody into host cells with reduced fucose addition capability, such as YB2/0 cells and cells lacking a fucose transporter. The anti-glypican 3 antibody with modified sugar chains of the present invention has a high level of cytotoxic activity and therefore is useful as a cell growth inhibitor such as an anticancer agent.本發明係關於糖鏈經改性抗磷脂醯肌醇蛋白聚糖3抗體,更具體為揭示岩藻糖欠缺之抗磷脂醯肌醇蛋白聚糖3抗體。本發明的抗磷脂醯肌醇蛋白聚糖3抗體可由上述糖鏈經修飾之抗體的製造方法所製造出,其為YB2/0細胞等岩藻糖加成能降低之宿主細胞或岩藻糖醇運子欠缺細胞上導入編碼抗磷脂醯肌醇蛋白聚糖3抗體之核酸,經培養該宿主細胞而製造。本發明的糖鏈改性抗磷脂醯肌醇蛋白聚糖3抗體具有高細胞障礙活性故可適用於抗癌劑等細胞增殖抑制劑上。
