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RESTORING FUNCTION TO A NON-FUNCTIONAL GENE PRODUCT VIA GUIDED CAS SYSTEMS AND METHODS OF USE
专利权人:
PIONEER HI-BRED INTERNATIONAL, INC.
发明人:
CIGAN ANDREW MARK,SVITASHEV SERGEI
申请号:
US201615765039
公开号:
US2018282763(A1)
申请日:
2016.10.17
申请国别(地区):
美国
年份:
2018
代理人:
摘要:
Compositions and methods are provided for restoring function to a non-functional gene product in the genome of a cell. The methods and compositions employ a guide polynucleotide/Cas endonuclease system to restore function to a non-functional gene product and to provide an effective system for modifying or altering target sites within the genome of a plant, plant cell or seed. The present disclosure also describes methods for modifying a nucleotide sequence in the genome of a cell using a restored functional selectable marker, as well as methods for editing a nucleotide sequence in the genome a cell without introducing a polynucleotide modification template into said cell. Compositions and methods are also provided for DNA free delivery of Cas endonucleases, sgRNAs and guide RNA/Cas complexes.
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中国工程科技知识中心
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