Provided is a method for producing an artificial recombinant virus of the family Reoviridae, the method comprising the steps of:(1) introducing a FAST protein expression vector and/or a capping enzyme expression vector into host cells；(2) introducing a vector containing expression cassettes for individual RNA genome segments of a virus or introducing a set of single-stranded RNA transcripts from the expression cassettes into host cells； and(3) culturing the host cells.The method of the present invention allows more efficient production of an artificial recombinant virus of the family Reoviridae as compared with conventional methods and allows artificial recombinant rotavirus production without using a helper virus.
