The present invention discloses a porous biological scaffold, wherein the porous bio scaffold is used to carry a plurality of stem cells, and includes a three-dimensional substrate and a plurality of holes. It is worth noting that the three-dimensional substrate is made of a high concentration aqueous solution of silk fibroin. And, the plurality of holes are connected to each other and have a pore diameter of from 100 micrometers to 1000 micrometers. Thus, the porous biological scaffold is paired with the plurality of stem cells to provide the patient with a biomedical structure that effectively promotes cell repair and proliferation.本新型係揭示一種多孔性生物支架,其中,該多孔性生物支架係用於攜載複數個幹細胞,且包括一立體基材以及複數個孔洞。值得說明的是,該立體基材由一高濃度的蠶絲蛋白水溶液所製成。並且,該複數個孔洞係彼此連通且其孔徑係介於100微米至1000微米。如此,該多孔性生物支架搭配該複數個幹細胞以提供患者一種有效地促進細胞修復與增生的生醫結構體。