Described herein are compositions comprising decellularized cardiacextracellular matrix and therapeutic usesthereof. Methods for treating, repairing or regenerating defective, diseased,damaged or ischemic cells, tissues or organs in a subject,preferably a human, using a decellularized cardiac extracellular matrix of theinvention are provided. Methods of preparingcardiomyocyte culture surfaces and culturing cells with absorbeddecellularized cardiac extracellular matrix are provided.