An ultrasound diagnostic apparatus includes an operating unit for inputting an instruction from an operator; and a controller for eliminating at least a part of the puncture guide line, which has been displayed so as to be superimposed on the ultrasound image, when an instruction for non-display of the puncture guide line is given through the operating unit during the freeze operation, and automatically returning the eliminated puncture guide line and displaying the eliminated puncture guide line so as to be superimposed on the real-time ultrasound image at the time of returning from the freeze operation to the live operation.