Refers to a method of purification of biologically active industrial phycotoxins such as neosaxitoxina, saxitoxin and gonyaulatoxina, comprising: (a) provide an amount of a source of phycotoxins such as cultivation of a clone of cyanobacteria, a pellet wet or frozen cyan Additive, among others (b) the materials resulting from the lysis of cyanobacteriaUndergoes a Cold Extraction and separation of organic and aqueous phases (Mixed chloroform: Methanol 1: 1 in 50% acetic acid and 1mm in 50% V \/ V, pH between 4 and 5) being the acid environment to maintain the Biological activity of the active ingredient (c) to obtain a Concentrate of the aqueous phase obtained and N the previous Stage or the stage of the Culture medium (a) using a rotor Vaporizer temperatureEnvironment (d) the concentrated supernatant centrifuged to obtain a Pass (e) the supernatant by a column of diatomaceous earth,Wash the column to obtain an eluate of phycotoxins with a solution of acetic acid ethanol: Water: 5mm 2: 1: 1 (f) The eluate obtained by Activated Carbon columns and eluted the phycotoxins (g) spend the eluate by a diatomaceous earth column and eluted the phycotoxins (H) Leave in aqueous Organic Solvents to evaporate the eluate prior to obtaining an extractFicotoxina of partially purified (i) to submit to the preparative HPLC Purified extract obtained ficotoxina pure. These phycotoxins are Cosmetic ApplicationSE REFIERE A UN METODO DE PURIFICACION INDUSTRIAL DE FICOTOXINAS BIOLOGICAMENTE ACTIVAS TALES COMO NEOSAXITOXINA, SAXITOXINA Y GONYAULATOXINA, QUE COMPRENDE: A) PROPORCIONAR UNA CANTIDAD DE UNA FUENTE DE FICOTOXINAS TAL COMO CULTIVO DE UN CLON DE CIANOBACTERIAS, UN PELLET HUMEDO O CONGELADO DE CIANOBACTERIAS, ENTRE OTROS B) EL MATERIAL PROVENIENTE DE LA LISIS DE CIANOBACTERIAS, SE SOMETE A UNA EXTRACCION EN FRIO Y SEPARACION DE FASES ORGANICA Y ACUOSA (MEZCLA CLOROFORMO:METANOL 1:1 EN 50% Y ACIDO ACETICO 1mM EN 50% V/V CON PH ENTRE 4 Y 5) SIENDO EL MEDIO ACIDO PARA MANTENER LA ACTIVI
