Method of producing and purifying an active soluble sialyltransferase

• 收藏
• 分享

专利权人:

发明人:

ANGERMANN AXEL,SCHECKERMANN CHRISTIAN,SCHMIDT KARSTEN

申请号：

IN1237/DELNP/2013

公开号：

IN2013DN01237A

申请日:

2013.02.08

申请国别(地区):

IN

年份:

2014

代理人:

摘要：

The present invention relates to a method for the production and purification of a sialyltransferase polypeptide in particular a N Acetylgalactosamine (Gal NAc) a 2 6 sialyltransferase I (ST6GalNAcI) polypeptide. The method comprises the steps of producing the sialyltransferase polypeptide in a Chinese Hamster Ovary (CHO) cell and purifying the polypeptide with a combination of chromatography steps. The method results in high yield of sialyltransferase polypeptide which is highly pure and active. The obtained sialyltransferase especially ST6GalNAcI can be employed for the glycosylation of therapeutic proteins such as G CSF.

来源网站：

中国工程科技知识中心

来源网址：

http://www.ckcest.cn/home/