The present invention is to provide an anti-splash needle mounting structure, which includes a grip portion having a receiving space therein for receiving a tattoo needle driving mechanism and an opening at a bottom end thereof in communication with the receiving space so that a tattoo needle of the tattoo needle driving mechanism can be exposed outside the opening, and a tattoo needle guide tube having two ends provided with two through holes respectively and an outer periphery of the top end thereof provided with a second connecting unit configured for connection with a first connecting unit provided in an inner periphery of the opening. The two through holes are in communication with each other, and the inner wall of the tattoo needle guide tube is axially formed with a plurality of grooves each having two ends extending to the two through holes respectively.
