The present invention pertains to a method for producing seeds of a Lactuca plant, the method including hybridizing the Lactuca plant using Megachile bees as a pollination means, and using as an auxiliary nectar source plant, in addition to the pollen parent Lactuca plant and the seed parent Lactuca plant, a plant that is preferentially visited by the Megachile bees, the visit preferentiality therefor being equal to or lower than that for the Lactuca plant. According to the present invention, seeds of the Lactuca plant can be produced more efficiently and economically than in past methods while the Megachile bees can be used as the pollination means.