The present invention relates to a tissue regenerative substance release-inducing cell therapeutic agent composition comprising: a medium for cryopreservation of animal cells that does not contain DMSO, Glycerol, and serum. . In the cell therapeutic agent of the present invention, the intracellular tissue regeneration promoting substance is released into the cryopreservation medium while the cell freezing and thawing process proceeds, and not only the animal cells but all the cryopreservation mediums of animal cells It is characterized by having a tissue regeneration effect. In addition, since the cell therapeutic agent of the present invention can be directly frozen and stored in a vial, ampoule or prefilled syringe, it is convenient for use, and since no cryopreservative and serum are used, it can be immediately transferred to the affected area without a separate washing step. Can be applied. [Selection] Figure 1本発明は、タンパク質、糖、緩衝液、及び基本培地；を含み、また、ＤＭＳＯ、Ｇｌｙｃｅｒｏｌ及び血清を含まない動物細胞の凍結保存用培地；を含む組織再生物質放出誘導型細胞治療剤組成物に関する。本発明の細胞治療剤は、細胞凍結及び解凍過程を進行しながら、細胞内組織再生促進物質が凍結保存用培地に放出され、且つ、動物細胞だけでなく、動物細胞の凍結保存用培地すべてが組織再生効能があることを特徴とする。また、本発明の細胞治療剤は、バイアル、アンプルまたはプレフィルドシリンジに直接凍結保管可能なので、使用便宜性が高くて、凍結保存剤及び血清を使用しないので、別途の洗浄段階なしに、直ちに患部への適用が可能である。【選択図】図１
