A biopsy guide for use in guiding a needle in a transperineal prostate biopsy procedure. The biopsy guide may include a guide member and a displacement member. The guide member may operably couple with the transrectal probe and include a guide body including a distal end, a proximal end, a length, a first guide member extending the length, and a second guide member extending the length, wherein a portion of the first and second guide members overhangs the transrectal probe when coupled thereto. The displacement member may be supported by the guide member and may include a central vertically extending member having a plurality of needle receiving ports therein, and a planar member coupled to the central vertically extending member and having a planar bottom surface that is configured to slide on the first and second planar top surfaces of the first and second guide members, respectively.