This invention relates to a manufacturing process of skin care product containing multiple growth factors, which is mainly proceeded as follows: firstly taking out healthy fat tissue then mixing a predetermined volume of solvent into the fat tissue for cleaning the fat tissue after that adding a predetermined amount of reagent into a predetermined volume of fat tissue and then performing centrifugal separation Mixing the produced fat tissue after being separated with a predetermined dosage of enzymes in a centrifugal tube, and performing oscillating to the centrifugal tube performing a cell culture process to the produced precipitate to produce somatic cells and culturing the obtained somatic cells to obtain growth factors with specific ratio. The cell culture medium obtained through the abovementioned manner contains abundant of growth factors (such as VEGF, HGF, b-FGF, TGF, IGF) secreted from cells, completing the manufacturing of raw material for high efficiency skin care products.本發明為有關於一種含有多種生長因子之保養品製程,其主要依下列方式進行:首先取出健康的脂肪組織;再將一預定容量的溶劑混入該脂肪組織,以對該脂肪組織進行清洗;按預定劑量的試劑加入預定容量之脂肪組織,並進行離心分離;經分離後產生的脂肪組織則混入預定劑量的酵素於一離心管,並對該離心管進行震盪;將產生的沉澱物進行細胞培養以產生體細胞;及取得該體細胞後,經由培養得出特定比例之生長因子,而透過上述方式所取得的細胞培養液富含細胞分泌之生長因子(如:VEGF、HGF、b-FGF、TGF、IGF),以完成高效保養品原料。
