An Engineered Transgene Integration Platform (ETIP) is described that can be inserted randomly or at targeted locations in plant genomes to facilitate rapid selection and detection of a GOI that is perfectly targeted (both the 5' and 3' ends) at the ETIP genomic location. One element in the subject disclosure is the introduction of specific double stranded breaks within the ETIP. In some embodiments, an ETIP is described using zinc finger nuclease binding sites, but may utilize other targeting technologies such as meganucleases, CRISPRs, TALs, or leucine zippers. Also described are compositions of, and methods for producing, transgenic plants wherein the donor or payload DNA expresses one or more products of an exogenous nucleic acid sequence (e.g.protein or RNA) that has been stably-integrated into an ETIP in a plant cell. In embodiments, the ETIP facilitates testing of gene candidates and plant expression vectors from ideation through Development phases.本文描述一種經工程處理的轉殖基因整合平台(ETIP)，其可被隨機地或於植物基因組中被標記之位置插入，用以促進快速篩選及偵測於該ETIP基因體位置被完整標記(包括5'及3'二端)之GOI。本說明書之要素為該ETIP中之該特異性雙鏈斷裂的引入。某些具體實施例描述使用鋅指核酸酶結合物之ETIP，但其可能利用其他標定技術，例如巨核酸酶(meganuclease)、CRISPRs、TALs或白胺酸鏈區。本文亦描述用於產生基因轉殖植物的組成物和方法，其中該供體或負載(payload)DNA表現一或多種之外源性核酸序列的產物(如蛋白質或RNA)，其具有穩定整合入植物細胞之ETIP。於具體實施例中，該ETIP促進測試發展階段中構思(ideation)的候選基因及植物表現載體。
