PROBLEM TO BE SOLVED: To provide a method for preparing a glycogen-containing composition by efficiently and easily concentrating glycogen from edible yeast.SOLUTION: This method for preparing the glycogen-containing composition by concentrating glycogen from edible yeast includes (a) a process of subjecting edible yeast to enzyme treatment with basidiomycete producing enzyme, (b) a process of microfiltrating an enzyme treated liquid produced in the (a) process followed by collecting filtration residue, and (c) a process of subjecting the filtration residue to centrifugal filtration followed by collecting glycogen-containing supernatant.COPYRIGHT: (C)2010,JPO&INPIT【課題】食用酵母から、効率的かつ簡便に、グリコーゲンを濃縮しグリコーゲン含有組成物を調製する方法を提供する。【解決手段】(a)食用酵母を、担子菌産生酵素類による酵素処理に供する工程;(b)工程(a)で生じた酵素処理液を精密濾過して濾過残渣を回収する工程;及び(c)濾過残渣を遠心分離に供し、グリコーゲンを含む上清を回収する工程を含む、食用酵母からグリコーゲンを濃縮してグリコーゲン含有組成物を調製する方法。【選択図】図2