Problem to be SolvedThe present invention provides a bioactivation method for enhancing a neural activity and/or a blood circulation activity of a living body and a hydrogen generating device for executing this method.SolutionAccording to this bioactivation method for enhancing a neural activity and/or a blood circulation activity of a living body, a gas mixture containing hydrogen and oxygen at predetermined concentrations is suctioned by spontaneous breathing continuously for a predetermined time. Moreover, this hydrogen generating device for executing the bioactivation method for enhancing a neural activity and/or a blood circulation activity of a living body includes a body cover member including a battery, a control substrate for controlling power supply from the battery, and a pair of positive/negative electrodes electrically conducted with or shut down from a positive electrode and a negative electrode of the battery by the control substrate, an electrolysis tank capable of storing water and detachably mounted on the body cover member and into which the pair of positive/negative electrodes are inserted therein in the mounted state, a nozzle portion having a through hole, and a mixing portion fluidically connecting the nozzle portion and an end portion of the electrolysis tank and having a channel for taking in the environmental air.