The present invention features nucleic acids for recombinant protein expression in mammalian cell culture. The episomal vectors of the invention promote high protein production in mammalian cells expressing the SV40 T Ag or Epstein-Barr virus nuclear antigen (e.g., COS7 or HEK293-6E cells). The methods and systems are useful, for example, in pharmaceutical drug development and cloning, especially for the production of antibodies.本發明之特徵在於在哺乳動物細胞培養物中表現重組蛋白之核酸。本發明之游離型(episomal)載體促進表現SV40 T Ag或愛-巴病毒(Epstein-Barr virus)核抗原之哺乳動物細胞(例如COS7或HEK293-6E細胞)中產生大量蛋白質。該等方法及系統可用於例如藥物開發及選殖,尤其用於產生抗體。(無元件符號說明)
