Goletz, Steffen,Danielczyk, Antje,Baumeister, Hans,Stahn, Renate,Löffler, Anja,Stöckl, Lars
申请号：
ES11176197
公开号：
ES2620261T3
申请日:
2007.09.10
申请国别(地区):
ES
年份:
2017
代理人:
摘要：
A method of selecting a host cell to produce a protein composition that has at least one of the following glycosylation characteristics: (i) has a number of G2 structures in the total carbohydrate structures or in the carbohydrate structures at a site of particular glycosylation of the protein molecule of said protein molecules in said protein molecular composition that is at least 5% higher compared to the same amount of protein molecules in at least one protein molecular composition of the same protein molecule isolated from ATCC No. CRL-9096 (CHOdhfr-) when expressed therein； and / or (ii) comprises more than 35% of G2 structures present in the total carbohydrate structures or in the carbohydrate structures at a particular glycosylation site of the protein molecule of the protein molecules in said protein composition； and / or (iii) has a number of G0 structures in the total carbohydrate structures or in the carbohydrate structures at a particular glycosylation site of the protein molecule of said protein molecules in said protein molecular composition that is at least 5% lower compared to the same amount of protein molecules in at least one protein molecular composition of the same protein molecule isolated from ATCC No. CRL-9096 (CHOdhfr-) when expressed therein； and / or (iv) comprises less than 22% of G0 structures present in the total carbohydrate structures or in the carbohydrate structures at a particular glycosylation site of the protein molecule of the protein molecules in said protein composition； and / or (v) comprises an amount of fucose in the total carbohydrate structures or in the carbohydrate structures at a particular glycosylation site of the protein molecule of said protein molecules in said protein molecular composition that is at least 5 % less compared to the same amount of protein molecules in at least one protein molecular composition of the same protein molecule isolated from ATCC No. CRL-9096 (CHOdhfr-) when expressed therein； and / or (
