Invention described herein provides high throughput method and reagent, its be used to generate transgenic animals (such as, non-human mammal), introduced by the multiple circulation (such as 4-10 circulations) via electroporation include a Cas9 protein a kind of CRISPR/Cas9 systems to gamete or transplant embryos early period (such as, single pond embryos or fertilized eggs), hereditarily lead to heritable modification of the genome of animal.
