The invention relates to the field of stem cell biology, in particular, to the field of line-specific differentiation of pluripotent or multipotent stem cells, which may include, but are not limited to: human embryonic stem cells (hESC, ESCH), in addition to non-embryonic induced pluripotent human stem cells (hiPS iPSCC), somatic stem cells, stem cells from diseased patients, or any other cells capable of line-specific differentiation. In particular, methods have been described for providing line-specific differentiation of ESCh and / or iPSCCH into progenitor cells of the ventral plate of the midbrain and then further into large populations of FOXA2 + LMX1A + TH + dopamine (DA) neurons of the midbrain using new culture conditions. FOXA2 + LMX1A + TH + dopamine (DA) neurons of the midbrain, obtained using the methods of the present invention, are further used for various purposes, including, but not limited to, use in in vitro analyzes for drug development and as a therapeutic agent for treating a disease, damage or lack of dopamine neurons in a patient. Further, compositions and methods are proposed for providing differentiation of FOXA2 + LMX1A + TH + dopamine (DA) neurons of the midbrain from human pluripotent stem cells for use in modeling diseases, in particular Parkinson's disease. In addition to natural DA, neurons contain a large number of markers, such as CD142, and neuronal cells of type A9.Изобретение относится к области биологии стволовых клеток, в частности, к области линиеспецифической дифференцировки плюрипотентных или мультипотентных стволовых клеток, которые могут включать, но не ограничиваются перечисленными: эмбриональные стволовые клетки человек (hESC, ЭСКч), помимо неэмбриональных индуцированных плюрипотентных стволовых клеткок человека (hiPSC, иПСКч), соматические стволовые клетки, стволовые клетки от больных пациентов или любые другие клетки, способные к линиеспецифической дифференцировке. В частности, описаны способы обеспечения линиеспецифич
