The present invention relates to a method for producing a cosmetic biocellulose sheet and to a cosmetic biocellulose sheet produced by using same. A biocellulose sheet is produced through a centrifuging and dehydrating step so as to have a water content of 40-70 wt%, a weight of 10-15 g , and a thickness of 0.1-0.2 mm, and the biocellulose sheet is dipped in a preservative solution including a water-soluble preservative that is antibacterial and is easily soluble in water, and is then processed in a centrifuging and dehydrating step once more, so that the cosmetic biocellulose sheet of the present invention is produced which has a water content of 90-98 wt%, a weight of 20-25 g, and a thickness of 0.25-0.5 mm. The cosmetic biocellulose sheet has antibacterial properties that are maintained for a long period of time, and can be stored at room temperature in contrast to a biocellulose sheet that is not treated in the preservative solution. By means of the present invention, the cosmetic biocellulose sheet can have antibacterial properties that are maintained for a long period of time even through a natural preservative is used , can be stored at room temperature for a long period of time even while being moved, and can use the effectively reduced amount of preservatives in contrast to general cosmetics when the cosmetic biocellulose sheet is used as a cosmetic product. [Reference numerals] (AA) Photo； (BB) Analysis； (CC) Control without microorganisms； (DD) Compression method (aqueous solution containing 0.5% of chitosan)； (EE) Centrifuging method (aqueous solution containing 0.1% of chitosan)； (FF) Centrifuging method (aqueous solution containing 0.5% of chitosan)； (GG) Decrease in preserving effect (about 200 cut of microorganisms detected)； (HH) Weal preservability (about 450 cut of microorganisms detected)； (II) Good preservability to reduce microorganisms (microorganisms not detected본 발명의 화장용 바이오셀룰로오스 시트의 제조방법 및 이를 이용하여 제조된 화장용 바이오셀룰로오스 시트에 관한 것이다.본 발명의 화장용 바이오
