RURAL DEVELOPMENT ADMINISTRATION);대한민국(농촌진흥청장);REPUBLIC OF KOREA(MANAGEMENT ;REPUBLIC OF KOREA(MANAGEMENT : RURAL DEVELOPMENT ADMINISTRATION)
发明人:
LEE, SU YOUNG,이수영,KIM, SEUNG TAE,김성태,LEE, EUN KYUNG,이은경,KIM, WON HEE,김원희,LEE, JUNG LIM,이정림,HAN, BONG HEE,한봉희
申请号：
KR1020100104647
公开号：
KR1020120043371A
申请日:
2010.10.26
申请国别(地区):
KR
年份:
2012
代理人:
摘要：
PURPOSE: A method for producing a large amount of transgenic plants using somatic cell embryo of Sweet Yellow is provided. CONSTITUTION: A method for producing a large amount of transgenic plants using rose 'Sweet Yellow' somatic cell embryo and embryogenic callus comprises: a step of co-culturing somatic cell embryo GUS gene-transformed Agrobacterium tumefaciens and GFP gene-transformed Agrobacterium tumefaciens； a step of culturing the co-cultured somatic cell embryo or embryogenic callues in an embryo selection medium containing 250mg/L^-1 of cefotaxim； a step of culturing the somatic cell embryo or embryogenic callus in a medium containing cefotaxim solely or together with PPT(phosphinothricin)； a step of culturing a cultured shoot in a medium； a step of culturing the shoot in a shoot growth medium； a step of culturing 1-1.5cm of shoots in a multi-shoot growth medium； and a step of culturing the multi-shoots in a root inducing medium.본 발명은 장미 품종 '스위트 엘로우(Sweet Yellow)'의 기내 식물체 뿌리에서 유도된 체세포배 및 배발생 캘러스를 재료로 아그로박테리움-매개 형절전환체를 대량생산할 수 있는 효율적인 유전자 전이법에 관한 것이다.
