PROBLEM TO BE SOLVED: To focus on a glycoprotein N-type sugar chain modification capable section, prepare an antibody recognizing a pancreas specific ribonuclease 1 (thereafter referred to as RNase 1), and to detect a pancreas cancer, using the antibody.SOLUTION: A monoclonal antibody is prepared which couples to a pancreas specific RNase 1 when a sugar chain is not coupled to an N-type sugar chain modification capable section of the pancreas specific RNase 1, and in which a coupling to the pancreas specific RNase 1 is prevented when the N-type sugar chain is coupled thereto, and a monoclonal antibody capable of simultaneously coupling to the pancreas specific RNase 1 together with the monoclonal antibody is prepared. Using these, a ratio of A to B is calculated to thereby detect a pancreas cancer, where the A denotes the N-type sugar chain modification capable section of the pancreas specific RNase 1 and an amount of the section having the N-type sugar chain coupled or non-coupled, and the B denotes an amount of the N-type sugar chain modification capable section of the pancreas specific RNase 1.【課題】糖タンパク質のＮ型糖鎖修飾可能部位に着目し、膵臓特異的リボヌクレアーゼ１（ＲＮａｓｅ １）を認識する抗体を作製し、それを用いて膵臓癌を検出する。【解決手段】膵臓特異的ＲＮａｓｅ １のＮ型糖鎖修飾可能部位に、糖鎖が結合していない場合に結合し、Ｎ型糖鎖が結合している場合に結合が阻害されるモノクローナル抗体を作製し、またこの抗体と同時に同膵臓特異的ＲＮａｓｅ １に結合できるモノクローナル抗体とを作製し、それらを用いて、ＡとＢとの比を求めることにより膵臓癌を検出する。Ａ＝膵臓特異的ＲＮａｓｅ １のＮ型糖鎖修飾可能部位であって、Ｎ型糖鎖が結合している部位又は結合していない部位の量。Ｂ＝膵臓特異的ＲＮａｓｅ １のＮ型糖鎖修飾可能部位の量。【選択図】 図１１
