PROBLEM TO BE SOLVED: To provide human pancreatic ribonuclease 1 (RNase 1) and a method for simply isolating the RNase 1.SOLUTION: An isolation method for RNase 1 comprises contacting an antibody specifically bindable to the RNase 1 in which one or more sites among three N-glycan chain modifiable sites in the RNase 1 are not modified with an N-glycan chain, and the RNase 1 in which no N-glycan chain is linked to the N-glycan chain modifiable sites in the RNase 1 with a specimen and then isolating the RNase 1 bound to the antibody.COPYRIGHT: (C)2015,JPO&INPIT【課題】ヒト膵臓特異的リボヌクレアーゼ1(RNase1)及び該RNase1を簡便に分離する方法の提供。【解決手段】RNase1の3つのN型糖鎖修飾可能部位のうち1つ以上の部位がN型糖鎖修飾を受けていないRNase1及びRNase1のN型糖鎖修飾可能部位にN型糖鎖が付加していないRNase1に対して特異的に結合可能な抗体と試料とを接触させ、次いで抗体に結合したRNase1を分離することを特徴とする該RNase1の分離方法。【選択図】図3
