VECTEUR D'EXPRESSION/REPLICATION SPECIFIQUE D'UN TYPE DE CELLULE CONCU A PARTIR DU VIRUS HERPES SIMPLEX COMPRENANT LE GENE ICP4 ET LE PROMOTEUR DU GENE DE LA CALPONINE HUMAINE
The present invention is to provide a method wherein acell-specific expression/replication vector that express andreplicate a gene specifically in specific cells such asmalignant tumors and the like and does not injure normal cells,particularly a vector that can suppress theexpression/replication at a desired period after theexpression/replication is constructed, f or the use in therapiesfor such as malignant tumors and the like, and treatment isconducted by introducing the vector to a particular living cellsuch as malignant tumor and the like for expression. Acell-specific expression/replication vector that does not actto adult normal cells is constructed by: a transcriptionalinitiation regulatory region of human calponin gene that isexpressed in smooth muscle cell specifically is obtained； saidregion is linked upstream of the replication-related gene ofvirus such as ICP4 and the like； a DNA that encodes proteinssuch as suppressive factor for tumor angiogenesis orapoptosis-related factors and the like is linked via IRES tosaid replication-related gene of the virus； and thyimidinekinase gene in an intact state is integrated into a viral DNA.This vector thus constructed is infected and introduced tomalignant tumor cells, and malignant tumor cells areselectively disrupted.
