A novel process for the formation of enzyme immobilized silica-alginate beads is reported perfectly suitable for clinical analysis of clinically significant analyte like blood glucose, cholesterol, urea, creatinine, acetylcholine, choline and dopamine. The invention relates to the stabilization of enzyme in silica-alginate beads in general and constitution of an efficient system of glucose detection in clinical assays in particular. Oxidases are immobilized into the calcium alginate matrix, in order to stabilize and enhance the enzyme activity, for its iterative use. The solution of enzyme (glucose oxidase) and the colloidal dispersion of Palladium/GO nanocomposite were gently mixed with the solution of sodium alginate, in appropriate amounts with thorough stirring at each step. The contents of the mixture are homogenized properly. The Palladium/GO nanocomposite was synthesized as reported previously (IP 201611028329). Further, for the fabrication of enzyme immobilised silica-alginate beads, the mixture was rapidly injected into the calcium chloride solution with continuous stirring. The spherical porous beads obtained are filtered and washed several times and stored at 4ºC for further use. Further, glucose substrates were taken in different samples tubes marked with the concentrations from high to low. The two porous beads immobilized enzymes (mentioned above), are added to each sample reactor and were stirred gently. The reaction is incubated for maximum 10 minutes, followed by the collection of sample from all the containers. Further, the solutions of p- hydroxybenzoicacid and 4-aminoantipyrine are mixed in equal ratio and a fixed volumes followed by adding appropriate amount of processable Prussian blue, which was made as reported earlier (IP 4043/DEL/2014), of resulting mixture are taken sample tubes, to which the above collected are added according to the respective concentrations and incubated for 15-20 minutes. After a persistent color is gene
