Sterilization tray fasteners are disclosed herein. The disclosed sterilization tray fasteners may be formed to join sterilization tray bases stacked directly on top of one another together. In some embodiments, sterilization tray bases may be formed to have slots or apertures to accommodate features of the disclosed fasteners. For example, in some embodiments, the disclosed sterilization tray fasteners include an elongate body and two curved arms that can extend into apertures of the sterilization tray bases. In many embodiments, the disclosed fasteners can be attached to and detached from sterilization tray bases without tools.