An animal waste collection device. An exemplary embodiment includes an elongated chassis defining a collection chamber having an open end, and a user-actuated pick up device with a movable shovel assembly mounted at the open end. The shovel assembly has a closed position for closing the open end, and an open position. A trap door is movable by the user between an opened position and a closed position located inside the collection chamber, to close the collection chamber in the closed position, and to open the collection chamber to entry of an animal waste specimen collected through the open end with the shovel assembly in the open position. Once the specimen is collected, the device may be inverted, the waste proceeding by gravity from the pick-up device, past the open trap door and into the collection chamber.
