The present invention relates to artificial ovulation and egg-laying induction methods for freshwater eels. A feminization inducer (for example, 17β-Estradiol) for inducing feminization is introduced into an aquaculture tank in which a parent fish to be artificially ovulated is raised for a certain period. When the time for inducing ovulation arrives, the aquaculture tank is mixed with flathead mullet Pituitary Extracts (FPE) as a man-made ovulation promoting substance. As a result, artificial ovulation is promoted without applying an administration method by intraperitoneal injection. Preferably, fresh water, brackish water, and seawater conditions are selectively set for the aquaculture water in the aquaculture tank, and the aquaculture water tank is configured to induce complete feminization of the freshwater eel parent fish in the freshwater conditions of the aquaculture water. A feminization inducer is introduced and a pretreatment process relating to feminization is performed. After this, the aquaculture water is set to seawater conditions, and the ovulation inducer is introduced into the aquaculture water. In this way, ovulation and egg laying of the parent fish are induced.淡水ウナギの人工排卵及び産卵誘導方法に関する。人工排卵対象の親魚が飼育される養殖水槽に、一定の期間にわたって、雌性化を誘導するための雌性化誘導物質（例えば、17β-Estradiol）を投入する。排卵誘導時期が到来すると、その養殖水槽に、人為的な排卵促進物質としての降河性魚類であるボラ（鯔）の脳下垂体抽出物（Flathead mullet Pituitary Extracts； FPE）を混在させる。これにより、腹腔注射による投与方式を適用せずとも人為的な排卵を促進させる。好ましくは、養殖水槽の養殖水については淡水、汽水及び海水条件の設定が選択的に行われ、養殖水の淡水条件で養殖水槽には前記淡水ウナギの親魚の完全な雌性化を誘導するための雌性化誘導物質を投入し、その雌性化に関する前処理過程の実行する。この後、養殖水を海水条件に設定し、排卵誘導物質を養殖水に投入する。このようにして、親魚の排卵及び産卵を誘導する。
