Apparatus for treating allografts, having a sonication tank (21) configured totransmit ultrasonic energy to the interior of the tank a treatment canister (40)rotatably positioned in said sonication tank (21) , and configured to receive allograftstherein and a treatment fluid source (60, 62, 64, 66) in fluid communication withsaid treatment canister (40) . Methods of treating allografts and methods fordetermining microbial contamination using the apparatus.