Apparatus for treating allografts, having a sonication tank (21) configured totransmit ultrasonic energy to the interior of the tank; a treatment canister(40) rotatably positioned in said sonication tank (21) , and configured toreceive allografts therein; and a treatment fluid source (60, 62, 64, 66) influid communication with said treatment canister (40) . Methods of treatingallografts and methods for determining microbial contamination using theapparatus.