The present disclosure relates to a method of detecting Chikungunya virus in a sample by a recombinant coat peptide antigen of Chikungunya virus. The disclosure further relates to the nucleotide and peptide sequences for arriving at the recombinant coat peptide of the disclosure. Further, the method of arriving at the recombinant peptide antigen is also described herein. Further, vector and host cells obtained in the present disclosure are also described herein, along with a kit to detect the said virus.