The ability to detect when a person is hiding important information has high value in many applications. A new class of systems, termed autonomous scientifically controlled screening systems (ASCSS), is designed to detect individuals purposely hidden information about target topics of interest. ASCSS represents a systematic synthesis of structured interviewing, orienting theory, defensive response theory, non-invasive psychophysiological measurement, and behavioral measurement. To evaluate and enhance the design principles, an automated screening kiosk (ASK) system was constructed. The ASK system has been used in a physical security screening scenario in which participants constructed and attempted to smuggle a fake improvised explosive device (IED). The ASK system results indicate that ASCSS enables more widespread application of credibility assessment screening systems.