Problem to be solved: to provide a color added natural black pigment which is useful as a hair coloring agent by natural material, and it is possible to use the color added squid brown color particles which have excellent color resistance, color resistance and heat resistance.A chemical compound having a carboxy group of melanin polymer and protein, which is the main component of squid colorimetric particles, as an active ester, and an organic molecule such as ethylenediamine having an amino group end and a positive charge (cation) terminal added thereto Squid additive squid ink.N-hydroxysuccinimide (NHS) and 1-ethyl-3 - (3-dimethylaminopropyl) carbodiimide hydrochloride (WC) in the acid buffer solution (pH 4.4 to 4.6) were reacted to squid coloured particles and squid A method for producing a cationic addition cuttlefish submicron particle which further reacts with an ethylenediamine (EDA) having an amino group end and a positive terminal at the carboxy group of the melanin polymer as a reactive NHS ester and reacts with an amide bond.Diagram【課題】天然素材による染毛剤として有用な、可食性天然黒色色素で優れた耐変色性、耐退色性及び耐熱性を有しているイカ墨色素粒子を正に帯電させたカチオン付加イカ墨色素粒子の提供。【解決手段】イカ墨色素粒子の主成分であるメラニン高分子及びタンパク質のカルボキシ基を活性エステルにし、アミノ基末端及び正電荷(カチオン)末端を併せ持つエチレンジアミン等の有機分子を付加して製造するカチオン付加イカ墨色素粒子。N-ヒドロキシコハク酸イミド(NHS)と1-エチル-3-(3-ジメチルアミノプロピル)カルボジイミド塩酸塩(WC)を酸性緩衝液液(pH4.4~4.6)中で、イカ墨色素粒子に反応させ、イカ墨色素粒子のメラニン高分子のカルボキシ基を反応性性のNHSエステルとし、アミノ基端及び正電荷末端を有するエチレンジアミン(EDA)を更に反応させ、アミド結合にて反応させる、カチオン付加イカ墨色素粒子の製造方法。【選択図】図2
