The present invention discloses an amino acid composition formula with moisture-retaining capacity, which is composed of alanine, Glutamic acid, serine, arginine and histidine in accordance with a fixed proportion, and applicable to related fields of food and skin care products to achieve its moisture-retaining capacity. When the amino acid composition formula is composed of 20wt% of alanine, 20wt% of Glutamic acid, 20wt% of serine, 20wt% of arginine, and 20wt% of Histamine, the moisture-retaining effect thereof is the best.本發明係揭露一種具有保濕能力之胺基酸組成配方,其係由丙胺酸(Alanine)、麩胺酸(Glutamic acid)、絲胺酸(Serine)、精胺酸(Arginine)及組胺酸(Histamine)依固定比例所組成,並可運用於食品及保養品之相關領域以達其保濕之能力。