An ultrasound diagnostic apparatus includes a monitor displaying an ultrasound image, an operating unit receiving an operating instruction from an operator and a control unit controlling a diagnostic apparatus body. The control unit causes measurement calipers each having a measurement line extending linearly in a vertical direction and a gate portion including an upper gate line and a lower gate line which are disposed parallel to each other on the measurement line to be displayed in a superimposed manner on the ultrasound image displayed on the monitor based on the operating instruction from the operating unit, and measurement is sequentially performed at more than one measurement site using the gate portion of each of the measurement calipers which is capable of rotation and change of the distance between the upper gate line and the lower gate line.