DAVIES JOHN P.,LIU XING L.,REDDY VAKA S.,AINLEY WILLIAM M.,WAGNER RY
申请号：
ARP110103137
公开号：
AR082785A1
申请日:
2011.08.29
申请国别(地区):
AR
年份:
2013
代理人:
摘要：
Methods for generating a marked population of maize plants include Transforming a cell or tissue of maize with the constructions disclosed.Claim 1: construction of DNA by marked Activation of maizeWhich comprises a sequence encoding a transposase； a region encoding a detectable Source comprising a sequence encoding a regulatory Gene of anthocyanin B - Peru a constitutive Promoter operatively linked First high level； and a sec Uence of anthocyanin Regulatory Gene that encodes a C1 Promoter operatively linked to a Second builtTitutivo high level； and a cassette transposition not Independent t-dnas inserted into the coding region of a detectable Source so that the genes B and C1 - peru express anthocyanins in a cell that contains the Construction of maize DNA by marked Activation of S Only after having Cut the cassette transpositionWhere the transposition cassette includes a pair of DNA Substrates for the transposase, which provides an Enhancer element of the transcript； and optionally a sequence encoding a selectable Marker linked operatively to the Enhancer element of the transcripci NG.Claim 12: the method of claim 1, wherein the identification of a marked population of maize plants includes Measuring the content of anthocyanin in a transformed plant Cell or tissue of maize, and compare the content of anthocyanin in the cell or you Jido Plant Maize transformed with a cell or tissue of maize Plant Control.Los métodos para generar una población marcada de plantas de maíz incluyen transformar una célula o tejido de maíz con las construcciones divulgadas.Reivindicación 1: Una construcción de ADN de activación por marcado de maíz, caracterizada porque comprende una secuencia de codificación de una transposasa； una región de codificación de un informante detectable que comprende una secuencia que codifica un gen regulador de antocianina B-Peru ligado operativamente a un primer promotor constitutivo de gran nivel； y una secuencia que codifica un gen regulador de antocianin
