William M. Ainley;Vaka S. Reddy;Xing Liang Liu;D. Ry Wagner;John P. Davies
发明人:
John P. Davies,Vaka S. Reddy,William M. Ainley,Xing Liang Liu,D. Ry Wagner
申请号：
US13220570
公开号：
US08912393B2
申请日:
2011.08.29
申请国别(地区):
US
年份:
2014
代理人:
摘要：
Disclosed herein is an activation tagging construct for maize, resulting tagged populations and plants. In one example, an activation tagging DNA construct includes a coding sequence for a transposase, a detectable reporter (such as anthocyanin regulatory genes B-Peru and C1) and a non-autonomous transposable T-DNA cassette. For example, the transposable T-DNA cassette is inserted into the detectable reporter encoding region such that the B-Peru and C1 genes express anthocyanins in a cell containing the maize activation tagging DNA construct only upon excision of the transposable cassette. Methods of generating a tagged population of maize plants include transforming a maize plant cell or tissue with the disclosed constructs.
