THE GOVERNMENT OF THE UNITED STATES OF AMERICA REPRESENTED BY THE SECRET ARY DEPARTMENT OF HEALTH AND HUMAN SERVICES
发明人:
LIU, KE,ROSENBERG, STEVEN A.
申请号：
CA2501087
公开号：
CA2501087C
申请日:
2002.10.15
申请国别(地区):
CA
年份:
2012
代理人:
摘要：
A method of preparing autologus T-lymphocytes for re-introduction into apatient having cancer, which method comprises obtaining peripheral bloodmononuclear cells (PBMCs) from a patient immunized with an antigen of thecanncer, stimulating the PBMCs with the antigen of the cancer in vitro,transducing the PBMCs with a retrovial vector, which (a) comprises andexpresses a human interleukin-2 (IL-2) coding sequence operably linked to aretroviral promoter, (b) does not comprise an exogenously introduced gene thatenables phenotypic selection, and (c) comprises a viral envelope thatefficiently transduces CD8+ T-lymphocytes； a method of preparing autologoustumor-infiltrating lymphocytes (TILs) for reintroduction into a patient havingcancer, which method comprises obtaining TILs from a tumor of the patient,optionally immunized with an antigen of the cancer, optionally stimulating theTILs with the antigen of the cancer in vitro, and transducing the TILs withthe aforementioned retroviral vector； compositions comprising cells obtainedin accordance with such methods； and methods of treating a patient havingcancer by administering to the patient cells obtained in accordance with suchmethods or compositions comprising same.
