PROBLEM TO BE SOLVED: To provide a surface plasmon enhanced fluorescence measuring apparatus capable of detecting fluorescence surely by removing a noise, even when each wavelength range of the fluorescence and excitation light is near.SOLUTION: A surface plasmon enhanced fluorescence measuring apparatus 1 has: a photodetector 14 for detecting light including fluorescence emitted from a fluorescent material (20) in a photon unit; a fluorescent filter 10 arranged in a detection optical path from a substrate (21) to the photodetector 14, for allowing passage of only light having a wavelength range of the fluorescence; and a dimmer filter 12 arranged in a detection optical path from the fluorescent filter 10 to the photodetector 14, for allowing passage of light in the whole wavelength, while reducing the light at a prescribed ratio.
