Provided herein are methods of producing, compositions comprising and uses ofoligodendrogenic neural progenitor cells(o-NPCs), made using a combination of PDGFR agonist and thyroxin or a thyroxinanalogue. The method includes； obtaining ventralizedneural progenitor cells (NPCs), the ventralized NPCs expressing Sox2, Nkx6-1,decreased level of Pax6 compared to unpatterned NPCs,and elevated expression of HoxA4 compared to unpatterned NPCs； culturing theventralized NPCs for about 12 to about 16 days (days26-40 of Fig. 7； days 12 to 27 of Fig. 10) in neural expansion media (NEM)supplemented with i) PDGFR agonist for the about 12to about 16 days and ii) thyroxine or a thyroxine analogue for the latterabout 7 to about 9 days, to produce o-NPC expressing Sox2and Nkx2.2, decresed level of Pax6 and Nkx6.1 compared to ventralized NPCs andelevated level of HoxA4 and Olig2 compared toventralized NPCs.
