PROBLEM TO BE SOLVED: To provide a production method of contrast agents for optical imaging containing a conjugate in which a hydrophobic dye is reacted with a ligand.SOLUTION: The invention provides a production method of contrast agents comprising a reaction step of allowing a hydrophobic dye, such as cyanine dye, to react with a ligand, such as polyethylene glycol and albumin, to obtain a conjugate and a purification step of allowing sorbents, such as aryl agarose, aryl organic polymer resin, alkyl agarose, alkyl organic dextran, and a resin having an acrylic group, to adsorb an unreacted hydrophobic dye in water.COPYRIGHT: (C)2016,JPO&INPIT【課題】疎水性色素とリガンドとを反応させた複合体を含む光学イメージング用造影剤の製造方法の提供。【解決手段】シアニン系色素等の疎水性色素とポリエチレングリコールやアルブミン等のリガンドとを反応させて複合体を得る反応工程と、未反応の前記疎水性色素を水中でアリールアガロース、アリール有機ポリマー樹脂、アルキルアガロース、アルキル有機デキストランおよびアクリル基を有する樹脂等の吸着剤に吸着させる精製工程と、を有する造影剤の製造方法。【選択図】なし
