The present invention is provided to the materials and methods that immune real estate is derived from the active antigen of the component of picornaviridae virus family from birth. Picornavirus antigen of the invention may be at the form as a kind of vaccine in therapeutic treatment or for preventing picornavirus infection from facilitating a main body. Picornavirus antigen of the invention can be in the shape for being administered for an immunogenic composition of the vaccine of the prevention of enterovirus infection. The present invention further comprises immunogenic composition, and it includes human enterovirus a, human enterovirus B, human enterovirus C, human enterovirus D antigens and their use in vaccine that being used for, which prevents enterovirus infection,.
