This invention disclosures a bio-sensing apparatus and sensing method thereof. The bio-sensing apparatus comprises at least one light sensor, reflection light module, transmission light module and control module, wherein the bio-sensing apparatus can provide two different light from the up and down side of the finger, and calculate the information of blood sugar by sensing the spectrum of light to provide the more precision measurement of the blood sugar concentration.一種生理感測裝置及其感測方法，可以經由手指偵測血糖資訊。生理感測裝置包括至少一光感測器、反射光源模組、穿透光源模組與控制模組，可以在手指上下兩方提供不同光譜的光線至手指並且透過感測光譜計算血糖資訊，藉此提供更準確的血糖感測。
