JULIAN EDWARD SALE,MICHAEL S. NEUBERGER,SARAH JANE CUMBERS
申请号：
PT91806836
公开号：
PT2204447T
申请日:
1999.10.08
申请国别(地区):
PT
年份:
2016
代理人:
摘要：
The invention reflates to a method for preparing an antibody-producing cell line capable of directed constitutive hypermutation of a specific nucleic acid region, comprising the steps of: a) screening a clonal cell population for V gene diversity: b) isolating one or more cells which display V gene diversity and comparing the rate of accumulation of mutations in the V genes and other genes of the selected cells； and c) selecting a cell in which the rate of V gene mutation exceeds that of other gene mutation.
