In the present invention, provided is a composition for inhibiting the formation of fat cells including 3,4`-dihydroxyflavone (DHF) as an active ingredient. In addition, the present invention relates to a method for inhibiting the expression of peroxisome proliferator-activated receptor (PPAR) gamma of cells manufactured by treating 3,4`-dihydroxyflavone in precursor fat cell. In the present invention, 3,4`-dihydroxyflavone has effects of reducing the activity of MEK/ERK and accordingly inhibiting the formation of fat cells. Therefore, 3,4`-dihydroxyflavone can be a new alternative for treating overweight or obesity.본 발명은 3,4`-다이하이드록시플라본을 유효성분으로 포함하는 지방세포형성 저해용 조성물에 관한 것이다.