A 3D medical microscope includes a control unit for controlling the operations of the microscope; a housing having a content chamber; and a first lens, a second lens, a reflective unit and a driving unit disposed in the content chamber. The first lens and the second lens are disposed coaxially. The reflective unit has a first reflective surface and a second reflective surface, and is located between the first and second lenses. The first reflective surface corresponds to the first lens for causing a light axis of the first lens to be deflected and projected toward a vertical direction. The second reflective surface corresponds to the second lens for causing a light axis of the second lens to be deflected and projected toward the vertical direction. The driving unit drives the reflective unit by a driving motor so as to displace the reflective unit along the vertical direction.一種3D醫療顯微鏡,包含有一控制顯微鏡所需運作的控制單元、一具有一內容室的殼體,及置於該內容室的一第一鏡頭、一第二鏡頭、一反射單元、一驅動單元。該第一鏡頭及第二鏡頭呈同軸設置,該反射單元具有一第一反射面及一第二反射面,且位於該第一、二鏡頭之間,該第一反射面對應於該第一鏡頭,促使該第一鏡頭之一光軸轉折而朝向一垂直方向投射,該第二反射面對應於該第二鏡頭,促使該第二鏡頭之一光軸轉折而朝向該垂直方向投射。該驅動單元是由一驅動馬達驅動該反射單元,使該反射單元沿著該垂直方向位移。