PROBLEM TO BE SOLVED: To provide an improved efficient method for inducing proliferation of monocytes derived from the peripheral blood, in particular, a method for inducing proliferation of monocytes derived from the peripheral blood with improved donor dependency, for example.SOLUTION: According to the present invention, a method comprises: introducing at least three genes, cMYC, BMI1, and BCL2 or LYL1 genes, into monocytes and expressing the genes in the monocytes to form monocytes having proliferative abilities. In a method for inducing proliferation of monocytes, the monocytes derived from the peripheral blood are fresh monocytes that are not cryopreserved, and more preferably monocytes derived from cancer patients. According to the present invention, a method for producing dendritic cells comprises the steps of: preparing monocytes having proliferation abilities by the method for inducing proliferation of monocytes； inducing differentiation of the monocytes into dendritic cells by culturing the monocytes having the proliferation abilities； and preparing antigen-presenting dendritic cells by loading the prepared dendritic cells with an arbitrary antigen or introducing a gene of an arbitrary antigen to produce antigen-presenting dendritic cells.SELECTED DRAWING: Figure 1【課題】効率の良い、改善された末梢血由来の単球の増殖誘導方法、特に、例えば、ドナー依存性が改善された末梢血由来の単球の増殖誘導方法の提供。【解決手段】ヒト末梢血由来の単球の増殖誘導方法であって、単球に、少なくとも以下の３つの遺伝子：ｃＭＹＣ、ＢＭＩ１、及び、ＢＣＬ２又はＬＹＬ１遺伝子を導入して発現させ、増殖能を有する単球を作製することを含む方法。前記末梢血由来の単球が凍結保存されていない新鮮な単球であることが好ましく、がん患者由来の単球であることがより好ましい、単球の増殖誘導方法。単球の増殖誘導方法により増殖能を有する単球を作製する工程と、前記増殖能を有する単球を培養することにより樹状細胞へと分化誘導する工程と、作製した樹状細胞を任意の抗原で負荷（ｌｏａｄ）して抗原提示樹状細胞を作製する工程又は、任意の抗原の遺伝子を導入して抗原提示樹状細胞を作製する工程と、を含む、樹状細胞作製方法。【選択図】図１
