The present invention aims to provide a microbial proliferation inhibitor that uses a substance that is stable to heat and has an antibacterial effect against various microorganisms as an active ingredient, a method for preventing microbial contamination, and a beverage. The present invention relates to a microbial proliferation inhibitor containing isoxanthohumol as an active ingredient, a method for preventing microbial contamination using the same, and a beverage.本發明係以提供一種以對熱呈穩定且對於各種微生物具有抗菌作用的物質作為有效成分之微生物增殖抑制劑、防止微生物污染之方法,及飲料為目的。本發明係有關於一種包含異黃腐醇作為有效成分的微生物增殖抑制劑、使用其之防止微生物污染之方法,及飲料。