A method of treatment of organ dysfunction using a supernatant containing molecules, cytokines and vesicles secreted from mesenchymal stem cells (MSC) from animals is provided. The MSC are cultured, and then centrifuged to separate them from the desired supernatant containing the molecules and vesicles that may be used for treatment. Treatment may be for dogs, cats, and similar animals. This treatment may be for any number of ailments including, but not limited to mitigation or reversal of complications from heart, kidney, liver, or other organ impairment.
